6I41
Co-crystal structure of human SPOP MATH domain (wild-type) and human BRD3 fragment
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-24 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.8731 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 61.508, 55.180, 42.606 |
Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
Resolution | 19.632 - 1.900 |
R-factor | 0.1902 |
Rwork | 0.188 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ivv |
RMSD bond length | 0.006 |
RMSD bond angle | 0.772 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.800 | 1.820 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmeas | 0.058 | |
Number of reflections | 13067 | 534 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 98.3 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 100 mM Tris pH 8.5, 200 mM NaCl, 25% (w/v) PEG3350 |