6I3V
x-ray structure of the human mitochondrial PRELID1 in complex with TRIAP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-31 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 127.312, 127.312, 177.390 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 93.642 - 1.980 |
R-factor | 0.2072 |
Rwork | 0.205 |
R-free | 0.24520 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.298 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.642 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 59554 | |
<I/σ(I)> | 19 | |
Completeness [%] | 99.0 | |
Redundancy | 19.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM CHES/ Sodium hydroxide pH 9.5 200 mM Sodium chloride 40% (v/v) PEG 300 |