6I3T
Crystal structure of murine neuroglobin bound to CO at 40 K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 40 |
| Detector technology | CCD |
| Collection date | 2010-04-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.056, 88.056, 110.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.040 - 2.000 |
| R-factor | 0.16989 |
| Rwork | 0.167 |
| R-free | 0.22175 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1q1f |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.467 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.630 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.236 |
| Number of reflections | 11287 | 1614 |
| <I/σ(I)> | 27.2 | 7.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M NA ACETATE, 2M NA FORMATE, PH 4.6 |
