6I3O
Crystal structure of DEAH-box ATPase Prp22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.530, 93.640, 218.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.060 - 3.250 |
R-factor | 0.2483 |
Rwork | 0.247 |
R-free | 0.26370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fa5 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.723 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 86.060 | 86.060 | 3.350 |
High resolution limit [Å] | 3.250 | 50.000 | 3.250 |
Rmerge | 0.113 | 0.016 | 1.060 |
Rmeas | 0.126 | 0.023 | 1.181 |
Total number of observations | 116904 | ||
Number of reflections | 22460 | 6 | 1859 |
<I/σ(I)> | 12.04 | 23.84 | 1.52 |
Completeness [%] | 99.5 | 66.7 | 96.3 |
Redundancy | 5.205 | 1.333 | 4.976 |
CC(1/2) | 0.998 | 0.657 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM Bis-Tris propane pH6.5, 400mM K-Thiocyanate, 20% PEG3350 |