6I3J
Bilirubin oxidase from Myrothecium verrucaria in complex with ferricyanide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 134.382, 203.846, 226.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.300 - 2.590 |
R-factor | 0.1618 |
Rwork | 0.160 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xll |
RMSD bond length | 0.009 |
RMSD bond angle | 1.350 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 2.670 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.144 | 0.858 |
Rmeas | 0.157 | 0.963 |
Rpim | 0.061 | 0.428 |
Number of reflections | 48305 | 4411 |
<I/σ(I)> | 13.5 | 2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 6.5 | 4.9 |
CC(1/2) | 0.994 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.1 | 291.15 | 14% (w/v) PEG 3350, 0.1 M succinic acid, protein concentration 25 mg/ml |