6I3H
Crystal structure of influenza A virus M1 N-terminal domain (G18A mutation)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.560, 35.580, 69.290 |
| Unit cell angles | 89.71, 84.40, 69.02 |
Refinement procedure
| Resolution | 21.370 - 1.900 |
| R-factor | 0.2072 |
| Rwork | 0.206 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cqe |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 23546 | 1731 |
| <I/σ(I)> | 9.69 | 2.15 |
| Completeness [%] | 94.6 | 93.6 |
| Redundancy | 1.96 | 1.95 |
| CC(1/2) | 0.997 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30% EDO_P8K, 0.1 M MB2 pH 7.5, 10% NPS |
