6I3B
Crystal structure of cPizza6-AYW, a circularly permuted designer protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.7500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.051, 70.442, 58.144 |
| Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
| Resolution | 39.675 - 1.000 |
| R-factor | 0.1541 |
| Rwork | 0.153 |
| R-free | 0.17480 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.260 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.063 | 1.208 |
| Rmeas | 0.068 | 1.314 |
| Rpim | 0.026 | 0.512 |
| Number of reflections | 200513 | 9930 |
| <I/σ(I)> | 14.4 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.25M Sodium chloride 0.1M HEPES pH7 25%(w/v) PEG6000 |
