6I33
Crystal structure of human glycine decarboxylase (P-protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-28 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.810, 123.510, 99.510 |
| Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
| Resolution | 46.080 - 2.300 |
| R-factor | 0.2129 |
| Rwork | 0.212 |
| R-free | 0.23549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lhd |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.493 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.079 | |
| Number of reflections | 90460 | 13232 |
| <I/σ(I)> | 12.3 | 1.3 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 4.5 | 4.6 |
| CC(1/2) | 0.990 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M HEPES pH 7, 6 % PEG3350, 10 mM DTT |
