6I2S

Crystal structure of the SucA domain of Mycobacterium smegmatis KGD (R802A) in complex with GarA, following 2-oxoglutarate soak

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	C 2 2 21
Unit cell lengths	141.270, 168.060, 101.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.200 - 2.400
R-factor	0.193
Rwork	0.192
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yic 2kfu
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.030	2.450
High resolution limit [Å]	2.400	2.400
Rmerge	0.075	0.840
Rpim	0.039	0.409
Number of reflections	46369
<I/σ(I)>	13.9
Completeness [%]	98.0
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM Hepes-Na pH 7.5, 47 % v/v MPD