6I2Q
Crystal structure of the wild-type SucA domain of Mycobacterium smegmatis KGD (alpha-ketoglutarate decarboxylase), in complex with GarA
Replaces: 2XT9Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 141.250, 167.846, 102.808 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.480 - 2.150 |
R-factor | 0.165 |
Rwork | 0.163 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yic |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.830 | 2.200 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.062 | 0.747 |
Rpim | 0.030 | 0.361 |
Number of reflections | 65695 | |
<I/σ(I)> | 16.6 | |
Completeness [%] | 98.9 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Hepes-Na pH 7.0, 50% MPD |