6I2G
ALFA-tag binding nanobody (NbALFA) bound to ALFA-tag peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2018-04-06 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.92 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.245, 32.379, 64.735 |
Unit cell angles | 90.00, 147.22, 90.00 |
Refinement procedure
Resolution | 50.330 - 1.500 |
R-factor | 0.1669 |
Rwork | 0.165 |
R-free | 0.19978 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vnv |
RMSD bond length | 0.013 |
RMSD bond angle | 1.857 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.360 | 1.540 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 17571 | |
<I/σ(I)> | 12.7 | |
Completeness [%] | 95.2 | |
Redundancy | 5.6 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | Crystal grew in a drop containing 100 nl of 100mM sodium citrate pH6.0, 2M ammonium sulphate, 4% v/v tert-Butanol, and 100 nl of the peptide-bound protein (10.1mg/ml NbALFA-ALFA peptide complex, 20mM HEPES pH7.4, 100mM NaCl, 10% glycerol). |