6I1P
Respiratory complex I from Thermus thermophilus with bound NADH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 96.110, 341.450, 263.890 |
Unit cell angles | 90.00, 100.52, 90.00 |
Refinement procedure
Resolution | 58.712 - 3.207 |
R-factor | 0.219 |
Rwork | 0.210 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hea |
RMSD bond length | 0.007 |
RMSD bond angle | 0.996 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13rc1_2961: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.712 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.157 | 2.644 |
Rpim | 0.112 | 1.911 |
Number of reflections | 260997 | 35816 |
<I/σ(I)> | 5.3 | 0.5 |
Completeness [%] | 95.5 | 90.1 |
Redundancy | 2.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 296 | 100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside |