6I19

Crystal structure of Chlamydomonas reinhardtii thioredoxin h1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96770
Spacegroup name	P 31 2 1
Unit cell lengths	48.760, 48.760, 143.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.420 - 1.378
R-factor	0.183
Rwork	0.181
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ep7
RMSD bond length	0.005
RMSD bond angle	0.768
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.420	1.427
High resolution limit [Å]	1.378	1.378
Number of reflections	41955
<I/σ(I)>	25.45
Completeness [%]	99.8
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM ammonium sulfate, 100 mM sodium cacodylate, pH 6.5, 30% PEG 8000