6I0U
Crystal structure of DmTailor in complex with U6 RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-20 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 60.783, 60.783, 167.216 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.546 - 2.001 |
R-factor | 0.2074 |
Rwork | 0.206 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6i0s |
RMSD bond length | 0.004 |
RMSD bond angle | 0.639 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.550 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 25042 | |
<I/σ(I)> | 17.95 | 1.11 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 19.7 | 20.6 |
CC(1/2) | 1.000 | 0.484 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 35% (v/v) glycerol ethoxylate, 90 mM sodium malonate, pH7.0 |