6I0N
Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa in complex with denuded glycan ended in anhNAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.133, 68.424, 38.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.920 - 1.400 |
| R-factor | 0.1577 |
| Rwork | 0.156 |
| R-free | 0.18390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i05 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.887 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.920 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.051 | 0.884 |
| Rpim | 0.018 | 0.308 |
| Number of reflections | 18010 | 1767 |
| <I/σ(I)> | 20.7 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.8 | 9.1 |
| CC(1/2) | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.15 M NaF and 16% (w/v) PEG3350 |
