6I0N

Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa in complex with denuded glycan ended in anhNAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	67.133, 68.424, 38.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.920 - 1.400
R-factor	0.1577
Rwork	0.156
R-free	0.18390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i05
RMSD bond length	0.005
RMSD bond angle	0.887
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.920	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.051	0.884
Rpim	0.018	0.308
Number of reflections	18010	1767
<I/σ(I)>	20.7	2.7
Completeness [%]	99.9	100
Redundancy	8.8	9.1
CC(1/2)		0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.15 M NaF and 16% (w/v) PEG3350