6I0D
Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.700, 328.000, 260.900 |
| Unit cell angles | 90.00, 100.40, 90.00 |
Refinement procedure
| Resolution | 29.867 - 3.600 |
| R-factor | 0.2197 |
| Rwork | 0.214 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hea |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.816 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.262 | 1.605 |
| Rpim | 0.243 | 1.523 |
| Number of reflections | 173452 | 25274 |
| <I/σ(I)> | 4 | 0.6 |
| Completeness [%] | 96.3 | 95.8 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201 |
