6I05
Crystal structure of RlpA SPOR domain from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.588, 68.765, 38.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.380 - 1.213 |
| R-factor | 0.1545 |
| Rwork | 0.153 |
| R-free | 0.17120 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.781 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.383 | 1.257 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.040 | 0.186 |
| Rpim | 0.026 | |
| Number of reflections | 24714 | 911 |
| <I/σ(I)> | 16.46 | 2.85 |
| Completeness [%] | 89.1 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.15 M NaF and 16% (w/v) PEG3350 |
