6HZI
Apo structure of TP domain from Burkholderia pseudomallei penicillin-binding protein 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 106.394, 106.394, 95.678 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.200 - 2.290 |
| R-factor | 0.19606 |
| Rwork | 0.196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uy7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.798 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.200 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmeas | 0.311 | |
| Number of reflections | 25371 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 25% (w/v) polyethylene glycol 3,350 and 0.3 M sodium formate |
