6HZG
BP0997, GH138 enzyme targeting pectin rhamnogalacturonan II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9787 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 55.298, 68.379, 220.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.100 - 1.600 |
R-factor | 0.156 |
Rwork | 0.154 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.618 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.390 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 100505 | |
<I/σ(I)> | 5.9 | 1.7 |
Completeness [%] | 90.5 | 84.7 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 16 % PEG 8000, 100 mM Tris pH 8.4, 9% Butanol |