6HZF
BP0997, GH138 enzyme targeting pectin rhamnogalacturonan II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97835 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.785, 106.207, 138.504 |
| Unit cell angles | 90.00, 99.44, 90.00 |
Refinement procedure
| Resolution | 49.550 - 1.950 |
| R-factor | 0.1744 |
| Rwork | 0.173 |
| R-free | 0.20799 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.698 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.550 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 144883 | |
| <I/σ(I)> | 19.7 | 2.5 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 12.7 | 10.8 |
| CC(1/2) | 0.999 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 30 mM Glutamate, 30 mM Alanine, 30 mM Glycine, 30 mM Lysine, 30 mM Serine, 100 mM Tris/Bicine pH 8.5, 8 % Ethylene glycol, 16 % PEG 8000 |
