6HZ3
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT E243G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2015-07-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.450, 129.070, 159.610 |
Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.150 |
R-factor | 0.2313 |
Rwork | 0.230 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.120 | 3.230 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 61001 | |
<I/σ(I)> | 5.6 | 2 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4 | 293 | PH 4, VAPOR DIFFUSION, TEMPERATURE 293K,12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4 |