6HYZ
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT K248C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-07-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.220, 132.939, 159.951 |
| Unit cell angles | 90.00, 101.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.050 |
| R-factor | 0.227 |
| Rwork | 0.226 |
| R-free | 0.23400 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 3.120 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 70102 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4 |
