6HYY

Human phosphoserine phosphatase with serine and phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97857
Spacegroup name	C 2 2 21
Unit cell lengths	49.140, 129.140, 155.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.947 - 1.566
R-factor	0.1961
Rwork	0.195
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nnl
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.947	1.622
High resolution limit [Å]	1.566	1.566
Rmerge	0.014	0.244
Rmeas	0.020	0.345
Rpim	0.014	0.244
Number of reflections	69622	6498
<I/σ(I)>	22.3
Completeness [%]	99.3	93.82
Redundancy	2	2
CC(1/2)	1.000	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.05 M CaCl2; sodium cacodylate 0.1 M pH 6.5; PEG 2000 20%