6HYW

THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Y119F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	193
Detector technology	PIXEL
Collection date	2016-10-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9771
Spacegroup name	C 1 2 1
Unit cell lengths	183.000, 132.240, 161.480
Unit cell angles	90.00, 103.25, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.193
Rwork	0.192
R-free	0.21200
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.340	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	85783
<I/σ(I)>	9.9
Completeness [%]	93.2
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.