6HYV
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Y119A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2016-12-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.810, 132.380, 159.650 |
| Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.2093 |
| Rwork | 0.209 |
| R-free | 0.22120 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.230 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 90262 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4 |
