6HYN
Structure of ATG13 LIR motif bound to GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 101.131, 101.131, 101.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.565 - 1.140 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.18530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.296 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.565 | 1.180 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.040 | 1.820 |
| Rmeas | 0.040 | 1.900 |
| Rpim | 0.010 | 0.540 |
| Number of reflections | 62488 | 6252 |
| <I/σ(I)> | 23.7 | 1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 17.9 | 12.3 |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M NaCl, 10% PEG 8000, 0.1M Na K phosphate 6.2 |
