6HYH

Crystal structure of MSMEG_1712 from Mycobacterium smegmatis in complex with Beta-D-Fucofuranose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	98
Detector technology	PIXEL
Collection date	2018-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	118.065, 118.065, 232.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.620 - 2.500
R-factor	0.2123
Rwork	0.211
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hbd
RMSD bond length	0.010
RMSD bond angle	1.389
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.485	2.806
High resolution limit [Å]	2.500	2.512
Number of reflections	28372
<I/σ(I)>	10.4
Completeness [%]	99.9
Redundancy	27.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	sodium chloride, magnesium chloride, Tris pH 7.5, PEG 1500