6HYF

Crystal structure of the third FNIII domain from rat beta4 integrin, a binding site for periaxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-23
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.996
Spacegroup name	P 21 21 21
Unit cell lengths	51.220, 52.840, 144.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.788 - 1.600
R-factor	0.1915
Rwork	0.190
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wtw
RMSD bond length	0.013
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.061	2.392
Rmeas	0.073	2.839
Number of reflections	51757	3746
<I/σ(I)>	8.8	0.6
Completeness [%]	98.3	98.6
Redundancy	3.3	3.3
CC(1/2)	0.998	0.166

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 3350, NH4NO3