6HYA
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2015-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.240, 133.320, 157.720 |
| Unit cell angles | 90.00, 100.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.390 |
| R-factor | 0.1852 |
| Rwork | 0.184 |
| R-free | 0.21240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.840 | 3.500 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmerge | 0.065 | 0.406 |
| Number of reflections | 50723 | |
| <I/σ(I)> | 4.9 | 1.4 |
| Completeness [%] | 99.1 | 92.6 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 293 | PH 4, VAPOR DIFFUSION, TEMPERATURE 293K;12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4. |
