6HY9

THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	193
Detector technology	CCD
Collection date	2015-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	C 1 2 1
Unit cell lengths	181.990, 134.360, 159.180
Unit cell angles	90.00, 101.47, 90.00

Refinement procedure

Resolution	20.000 - 2.950
R-factor	0.204
Rwork	0.203
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	BUSTER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	3.010
High resolution limit [Å]	2.950	2.950
Rmerge	0.064
Number of reflections	76643
<I/σ(I)>	11.7
Completeness [%]	97.0	95.6
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4	291	PH 4, VAPOR DIFFUSION, TEMPERATURE 291K. 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.