6HY5
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2015-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.420, 133.490, 161.010 |
| Unit cell angles | 90.00, 102.18, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.580 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.640 | 2.620 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Number of reflections | 483142 | |
| <I/σ(I)> | 7.9 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 291 | 12-15% PEG4K, 0.1M NA ACETATE PH4, 0.4M NASCN, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K |
