6HX9

Putrescine transaminase from Pseudomonas putida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-11-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	62.550, 96.120, 151.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.450 - 2.050
R-factor	0.2092
Rwork	0.207
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ti8
RMSD bond length	0.007
RMSD bond angle	0.898
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASES
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.452	2.120
High resolution limit [Å]	2.050	2.050
Rmeas	0.106
Number of reflections	57573	2292
<I/σ(I)>	9.5
Completeness [%]	99.0
Redundancy	3.6
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M MgCl2, 0.1 M Bis-Tris pH 6.5, 25% PEG3350