6HVL
CdaA complex with c-di-AMP and AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 180 |
Detector technology | PIXEL |
Collection date | 2017-06-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976200 |
Spacegroup name | H 3 2 |
Unit cell lengths | 121.900, 121.900, 141.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.271 - 2.800 |
R-factor | 0.1899 |
Rwork | 0.188 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rv7 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.271 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 42.271 | 2.800 |
Rmerge | 0.109 | 0.020 | 0.805 |
Rmeas | 0.118 | 0.023 | 0.877 |
Number of reflections | 9435 | 2 | 941 |
<I/σ(I)> | 12.39 | 31.17 | 1.93 |
Completeness [%] | 93.1 | 66.7 | 95.4 |
Redundancy | 5.649 | 3 | 5.682 |
CC(1/2) | 0.998 | 0.726 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 m Ca(CH3OO)2, 0.1 M Na-HEPES pH 7.5, 10 % w/v PEG8000, |