6HV6
Crystal structure of PatoxP, a cysteine protease-like domain of Photorhabdus asymbiotica toxin PaTox
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.2037 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.403, 76.029, 76.324 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.864 - 2.001 |
R-factor | 0.1758 |
Rwork | 0.175 |
R-free | 0.21440 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.738 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.900 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.261 | 2.298 |
Number of reflections | 25589 | 1856 |
<I/σ(I)> | 14.7 | 2.5 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 95.5 | 62 |
CC(1/2) | 0.997 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 25.7% w/v Polyethylene glycol 8000, 150 mM HEPES pH 7.5, 60 mM potassium sodium tartrate |