6HUL

Sulfolobus solfataricus Tryptophan Synthase AB Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	80
Detector technology	CCD
Collection date	2009-05-30
Detector	MAR CCD 130 mm
Wavelength(s)	0.9184
Spacegroup name	P 65 2 2
Unit cell lengths	128.980, 128.980, 217.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.461 - 2.550
R-factor	0.1852
Rwork	0.182
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n2p 6hte
RMSD bond length	0.008
RMSD bond angle	1.034
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.461	2.641
High resolution limit [Å]	2.500	2.500
Rmerge	0.149
Rmeas	0.162
Rpim	0.061
Number of reflections	37260
<I/σ(I)>	11.85
Completeness [%]	99.5
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10 mM potassium phosphate pH 7.5, 100 mM KCl, 50 mM L-serine, 50 mM G3P