6HUA
the competence regulator ComR from Streptococcus vestibularis in complex with its cognate signaling peptide XIP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978570 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.190, 84.030, 152.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.873 - 3.389 |
R-factor | 0.2477 |
Rwork | 0.245 |
R-free | 0.30100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jub |
RMSD bond length | 0.002 |
RMSD bond angle | 0.493 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.590 |
High resolution limit [Å] | 3.389 | 3.390 |
Rmeas | 0.349 | |
Number of reflections | 11266 | 1748 |
<I/σ(I)> | 15.16 | 1.51 |
Completeness [%] | 99.7 | 98.5 |
Redundancy | 70.99 | 70.87 |
CC(1/2) | 0.999 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 290 | 12% PEG 4000, 0.1 M MgCl2, 0.1 M NaCl, 0.1 M tri-sodium citrate, 10 mg/ml protein concentration |