6HU8
Apo form of the competence regulator ComR from Streptococcus vestibularis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.070, 38.290, 87.620 |
| Unit cell angles | 90.00, 113.20, 90.00 |
Refinement procedure
| Resolution | 36.801 - 1.894 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5juf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.894 | 1.894 |
| Rmeas | 0.090 | |
| Number of reflections | 26937 | 4162 |
| <I/σ(I)> | 11.8 | 1.05 |
| Completeness [%] | 99.1 | 95.5 |
| Redundancy | 6.66 | 5.5 |
| CC(1/2) | 0.998 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 290 | 20% PEG 3350, 0.15 M DL-malic acid, 20 mg/ml protein concentration |
