6HTW

Crystal structure of an ancient sequence-reconstructed Elongation factor Tu (node 184)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 2
Unit cell lengths	82.203, 106.122, 112.472
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	112.470 - 1.800
R-factor	0.199
Rwork	0.197
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efc
RMSD bond length	0.020
RMSD bond angle	2.037
Data reduction software	XDS
Data scaling software	Aimless (0.5.23)
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	112.470	49.690	1.830
High resolution limit [Å]	1.800	9.850	1.800
Rmerge	0.055	0.027	1.819
Rmeas	0.060	0.030	1.993
Rpim	0.025	0.012	0.805
Number of reflections	91686	656	4407
<I/σ(I)>	13.9
Completeness [%]	99.8	98.9	97.8
Redundancy	5.9	5.5	6
CC(1/2)	0.999	0.999	0.425

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	10% PEG 8000, 20% ethylene glycol, 20 mM each of 1,6-hexanediol, 1-butanol 1-2-propanediol 2-propanol 1,4-butaediol 1,3-propanediol and 0.1 mM MES/imidazole, pH6.5