6HTV
Crystal structure of Leuconostoc citreum NRRL B-1299 N-terminally truncated dextransucrase DSR-M in complex with isomaltotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 185.308, 185.308, 150.774 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.960 - 3.900 |
| R-factor | 0.21125 |
| Rwork | 0.210 |
| R-free | 0.23209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lfc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 4.110 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 26662 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 97.3 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 3350 20 %, 0.2 M Sodium citrate tribasic dihydrate, 0.1 M Bis-Tris propane 6.5 |
