6HTU

Structure of hStau1 dsRBD3-4 in complex with ARF1 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-04-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.045
Spacegroup name	P 41 21 2
Unit cell lengths	105.886, 105.886, 169.221
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.354 - 2.888
R-factor	0.218
Rwork	0.217
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASES
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.888	2.888
Number of reflections	41096
<I/σ(I)>	20.52
Completeness [%]	99.4
Redundancy	27.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	50 mM MgCl2, 120 mM KCl, 50 mM Sodium cacodylate pH 6.33, 5% 1,6-Hexanediol