6HTJ
Crystal structure of the translation recovery factor Trf from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.291, 58.493, 101.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.304 - 1.650 |
| R-factor | 0.1923 |
| Rwork | 0.192 |
| R-free | 0.20710 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.304 | 1.709 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.145 | 1.238 |
| Rmeas | 0.150 | 1.292 |
| Rpim | 0.041 | 0.363 |
| Number of reflections | 29528 | 2817 |
| <I/σ(I)> | 13.32 | |
| Completeness [%] | 99.8 | 98.26 |
| Redundancy | 13.5 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.5 | 277.5 | 50 mM Bis Tris pH 6.5, 50 mM NaCl, 5 mM beta-mercapthoethanol |
