6HSY
Two-phospholipid-bound crystal structure of the substrate-binding protein Ttg2D from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.980227 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 124.639, 124.639, 38.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 62.320 - 2.530 |
R-factor | 0.2113 |
Rwork | 0.209 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Poly-A homology model based on 2QGU |
RMSD bond length | 0.003 |
RMSD bond angle | 0.473 |
Data reduction software | iMOSFLM (1.0.7) |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER (2.5.1) |
Refinement software | PHENIX (1.14rc1_3161) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.320 | 2.640 |
High resolution limit [Å] | 2.530 | 2.530 |
Rmerge | 0.124 | 0.707 |
Rmeas | 0.135 | 0.795 |
Rpim | 0.053 | 0.352 |
Number of reflections | 11489 | 1349 |
<I/σ(I)> | 8.2 | 2 |
Completeness [%] | 99.6 | 98 |
Redundancy | 6.2 | 4.6 |
CC(1/2) | 0.996 | 0.717 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.17 M Ammonium sulfate, 25.5% w/v PEG 4000, 15% v/v Glycerol |