6HS5

N-terminal domain including the conserved ImpA_N region of the TssA component of the type VI secretion system from Burkholderia cenocepacia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.04434
Spacegroup name	P 21 21 2
Unit cell lengths	49.670, 125.740, 45.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.210 - 1.800
R-factor	0.17966
Rwork	0.177
R-free	0.22594
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.567
Data reduction software	xia2
Data scaling software	xia2
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.670	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.043	0.344
Rpim	0.022	0.177
Number of reflections	27244	1966
<I/σ(I)>	21.2	4.4
Completeness [%]	99.8	99
Redundancy	5.4	5.3
CC(1/2)	0.999	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.16M calcium acetate, 0.08M sodium cacodylate pH6.5, 14.4% (w/v) PEG8000, 20% (v/v) glycerol