6HRS
Structure of the TRPML2 ELD at pH 4.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.523, 142.473, 85.195 |
Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
Resolution | 28.130 - 2.950 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hrr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.610 | 3.090 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.144 | 1.937 |
Rpim | 0.058 | 0.749 |
Number of reflections | 35855 | 4798 |
<I/σ(I)> | 9.8 | 1 |
Completeness [%] | 98.9 | 99.7 |
Redundancy | 7.2 | 7.6 |
CC(1/2) | 0.997 | 0.411 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 25% PEG 550 MME 0.1 M Na-Acetate pH 4.5 |