6HRS
Structure of the TRPML2 ELD at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.523, 142.473, 85.195 |
| Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
| Resolution | 28.130 - 2.950 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hrr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.610 | 3.090 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.144 | 1.937 |
| Rpim | 0.058 | 0.749 |
| Number of reflections | 35855 | 4798 |
| <I/σ(I)> | 9.8 | 1 |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 7.2 | 7.6 |
| CC(1/2) | 0.997 | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 25% PEG 550 MME 0.1 M Na-Acetate pH 4.5 |
