6HRN
C-Phycocyanin from heterocyst forming filamentous cyanobacterium Nostoc sp. WR13
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 63 |
Unit cell lengths | 151.473, 151.473, 39.764 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.750 - 1.513 |
R-factor | 0.11678 |
Rwork | 0.115 |
R-free | 0.15396 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | not published yet |
RMSD bond length | 0.012 |
RMSD bond angle | 1.612 |
Data reduction software | XDS (20180808) |
Data scaling software | pointless (1.11.14) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 131.179 | 1.589 |
High resolution limit [Å] | 1.513 | 1.513 |
Rmerge | 0.033 | 1.013 |
Rmeas | 0.037 | 1.135 |
Rpim | 0.016 | 0.470 |
Number of reflections | 73328 | 3665 |
<I/σ(I)> | 20.5 | 1.5 |
Completeness [%] | 94.6 | 54 |
Redundancy | 5.6 | 5.6 |
CC(1/2) | 1.000 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Morpheus crystallisation screen condition C4: 37.5% MPD_Peg1K_Peg3350 precipitant, 0.1M buffer system (imidazole & MES) and the NPS (nitrate, phosphate & sulfate) mix of additives. |