6HRL

Crystal structure of the Kelch domain of human KLHL17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	67.353, 118.774, 124.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.495 - 2.600
R-factor	0.2292
Rwork	0.227
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nkp
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.495	62.480	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.188	0.067	0.809
Number of reflections	31573
<I/σ(I)>	6.4
Completeness [%]	99.9	99.8	99.4
Redundancy	6.1	5.2	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.26M ammonium sulfate, 0.2M lithium sulfate, 0.1M tris pH 8.5