6HR0

Optimizing electroactive organisms: the effect of orthologous proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-30
Detector	PSI PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 43 21 2
Unit cell lengths	66.171, 66.171, 43.236
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.270 - 1.040
Rwork	0.152
R-free	0.17780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m1p
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.270	1.070
High resolution limit [Å]	1.040	1.040
Number of reflections	44836	2412
<I/σ(I)>	14.2
Completeness [%]	96.1
Redundancy	6.7
CC(1/2)	0.990	0.386

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Bicine pH 8.5 and 3.7 M Ammonium Sulfate