6HQJ
apo-form of polyphenol oxidase from Solanum lycopersicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-25 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9660 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.800, 54.140, 69.770 |
Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
Resolution | 42.468 - 1.802 |
R-factor | 0.1655 |
Rwork | 0.164 |
R-free | 0.20060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hqi |
RMSD bond length | 0.007 |
RMSD bond angle | 0.854 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.470 | 1.870 |
High resolution limit [Å] | 1.800 | 1.800 |
Rpim | 0.049 | 0.441 |
Number of reflections | 40854 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 99.0 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM sodium citrate pH 6.6, 13% PEG 8000 |