6HQI
holo-form of polyphenol oxidase from Solanum lycopersicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-01 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.620, 54.100, 69.820 |
Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
Resolution | 42.231 - 1.850 |
R-factor | 0.1928 |
Rwork | 0.191 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ce9 |
RMSD bond length | 0.017 |
RMSD bond angle | 0.876 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.231 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 37575 | |
<I/σ(I)> | 6.81 | |
Completeness [%] | 100.0 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM sodium citrate pH 6.6, 13% PEG 8000 |