6HQB

Monomeric cyanobacterial photosystem I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	82
Detector technology	PIXEL
Collection date	2017-09-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999
Spacegroup name	P 21 21 21
Unit cell lengths	124.322, 178.658, 181.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.289 - 4.000
R-factor	0.256
Rwork	0.255
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.299
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3228: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	4.000	4.000
Rpim	0.092	0.437
Number of reflections	34852
<I/σ(I)>	4.9
Completeness [%]	99.7
Redundancy	24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	KCl, MES-NaOH, PEG 3350, sulfoquinovosyldiacylglycerol or Jeffamine M-600
1	VAPOR DIFFUSION, SITTING DROP		277	KCl, MES-NaOH, PEG 3350, sulfoquinovosyldiacylglycerol or Jeffamine M-600